Copper Oxide Nanoparticle Diameter Mediates Serum?Sensitive Toxicity in BEAS?2B Cells

Copper oxide (CuO) nanoparticles (NPs) are abundant in manufacturing processes, but they an airway irritant. In vitro pulmonary toxicity of CuO NPs has been modeled using cell lines such as human bronchial epithelial line BEAS-2B. 2D culture, BEAS-2B undergoes squamous differentiation due to the presence serum. Differentiation is part repair process lung cells vivo that helps preserve lining respiratory tract. Herein, effects serum on hydrodynamic diameter, cellular viability, differentiation, and uptake 5 35 nm investigated, mean area used marker for cells. The results demonstrate diameter decreases with addition culture medium. Serum also increases area, only affects dose-dependent cytotoxicity NPs, while simultaneously having no effect intracellular Cu2+. This study presents evidence both NP size media influence relative viability following exposure highlights a critical need carefully designed experiments accurately reported conditions.